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mouse monoclonal anti lamp1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal anti lamp1
    Mouse Monoclonal Anti Lamp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lamp+1/LAMP-1+Antibody/pmc13016079-12-0-4
    Average 96 stars, based on 1986 article reviews
    mouse monoclonal anti lamp1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Immunogenicity and plasmid delivery pathways of non-invasive Lactococcus lactis -vectored mucosal DNA vaccination.
    Article Snippet: .. The cells were washed with PBS, fixed with 4% PFA for 15 min at room temperature, stained with anti-Lamp-1 (clone 1D4B; Santa Cruz Biotechnology) and anti-rat IgG-Alexa 594 (Thermo Fisher Scientific), or fixed with methanol for 5 min at −20°C, stained with anti-EGFP polyclonal antibody (MBL), anti-rabbit IgG-Alexa 594 (Thermo Fisher Scientific), and Hoechst 33342, and mounted with ProLong Gold antifade reagent (Thermo Fisher Scientific). .. Images were obtained using an LSM900 confocal microscope (ZEISS).

    Article Title: Immunogenicity and plasmid delivery pathways of non-invasive Lactococcus lactis -vectored mucosal DNA vaccination
    Article Snippet: .. The cells were washed with PBS, fixed with 4% PFA for 15 min at room temperature, stained with anti-Lamp-1 (clone 1D4B; Santa Cruz Biotechnology) and anti-rat IgG-Alexa 594 (Thermo Fisher Scientific), or fixed with methanol for 5 min at −20°C, stained with anti-EGFP polyclonal antibody (MBL), anti-rabbit IgG-Alexa 594 (Thermo Fisher Scientific), and Hoechst 33342, and mounted with ProLong Gold antifade reagent (Thermo Fisher Scientific). .. Images were obtained using an LSM900 confocal microscope (ZEISS).

    Article Title: Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunity.
    Article Snippet: .. Cells were fixed and permeabilized with ice- cold methanol, blocked with Blocking One Hist, and stained with biotin- conjugated goat anti- IgM F(ab′)2 (Southern Biotech) and Alexa 488–labeled anti- LAMP- 1 (Santa Cruz) at 4°C overnight. ..

    Article Title: Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunity
    Article Snippet: .. Cells were fixed and permeabilized with ice-cold methanol, blocked with Blocking One Hist, and stained with biotin-conjugated goat anti-IgM F(ab′) 2 (Southern Biotech) and Alexa 488–labeled anti-LAMP-1 (Santa Cruz) at 4°C overnight. ..

    other:

    Article Title: Subcellular localization of the P2X4 receptor in sensory hair cells of Wistar rat cochlea
    Article Snippet: Anti-LAMP-1, mouse IgG monoclonal , Santa Cruz Biotechnology Inc. (sc-20011) , Adherent spleen cells of human origin , 1:200 , Mouse, rat and human , Lysosomes , (Spangenberg et al., ), (Oh et al. ) .

    Article Title: Methods of treating Danon disease
    Article Snippet: Cells were washed 3 times with DPBS and then incubated with secondary antibodies (Molecular Probes Alexa Fluor 488, 1:400 or Alexa Fluor 555, 1:1,000; Abcam Alexa Fluor 647, 1:400) and Hoechst (Molecular Probes, 1:5,000) for 30 minutes at room temperature.

    Incubation:

    Article Title: G3BP1 Maintains Lysosomal Homeostasis to Limit Tau Aggregate Accumulation
    Article Snippet: .. Cells were incubated with anti-LAMP-1 (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) or anti-TIA-1 (1:1000 dilution; ProteinTech) antibody for 12 h at 25°C. .. After a PBS wash, cells were further incubated with Alexa Fluor Plus 488-labeled goat anti-mouse IgG or Alexa Fluor Plus 488-labeled goat anti-rabbit IgG (Thermo Fisher) for 4 h at 25°C.

    Blocking Assay:

    Article Title: Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunity.
    Article Snippet: .. Cells were fixed and permeabilized with ice- cold methanol, blocked with Blocking One Hist, and stained with biotin- conjugated goat anti- IgM F(ab′)2 (Southern Biotech) and Alexa 488–labeled anti- LAMP- 1 (Santa Cruz) at 4°C overnight. ..

    Article Title: Critical roles of chronic BCR signaling in the differentiation of anergic B cells into age-associated B cells in aging and autoimmunity
    Article Snippet: .. Cells were fixed and permeabilized with ice-cold methanol, blocked with Blocking One Hist, and stained with biotin-conjugated goat anti-IgM F(ab′) 2 (Southern Biotech) and Alexa 488–labeled anti-LAMP-1 (Santa Cruz) at 4°C overnight. ..



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    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and <t>LAMP1</t> (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
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    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and <t>LAMP1</t> (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
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    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and <t>LAMP1</t> (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
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    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and <t>LAMP1</t> (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
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    Image Search Results


    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Journal: eLife

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    doi: 10.7554/eLife.101533

    Figure Lengend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

    Techniques: Expressing, Staining

    ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Journal: eLife

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    doi: 10.7554/eLife.101533

    Figure Lengend Snippet: ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

    Techniques: Expressing, Staining, Immunofluorescence, Marker